Green Synthesis of Silver Nanoparticles Using Moringa oleifera sp. (Malunggay) Seed Aqueous Extract and Its Antibacterial Activity

Kim Dale Dogeno1, Gian Francesco Gamboa1, Luis Alfonso Pefianco1, Athenes Joy Aban1 and Aris Larroder1
1Philippine Science High School Western Visayas Campus – Bito-on, Jaro, Iloilo City 5000, Department of Science and Technology, Philippines


Abstract
Silver nanoparticles, in recent years have gained interest due to their applications in various fields, such as in medicine, as a result of its antibacterial properties. The synthesis of nanoparticles involves reduction and capping processes. The current processes, however, involve the use of toxic chemicals. A solution for this is the use of green synthesis, which is done through the utilization of biological components which acts as both a reducing and capping agent. The study used M. oleifera sp. seed extract to synthesize silver nanoparticles as they possess various biomolecules that make the process effective. Ultraviolet-Visible spectrophotometry was used to monitor the formation of the silver nanoparticles. The synthesized silver nanoparticles were viewed under a Transmission Electron Microscope and had a mean size of 12 nm and spherical shape. The silver nanoparticles also showed antibacterial activity against the S. aureus bacterial culture. The study concluded that silver nanoparticles can be synthesized using M. oleifera sp. seed extract.

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