Assessment of the Combination of Anthocyanin and Quercetin as an Angiogenic Inhibitor through a Chick Embryo Chorioallantoic Membrane Assay

Alexandria Marie Sombiro1, Daphne Di Valdestamon1, Kyle Dominic Aguirre1 and Harold Mediodia1
1Philippine Science High School Western Visayas Campus – Bito-on, Jaro, Iloilo City 5000, Department of Science and Technology, Philippines


Abstract
Angiogenesis is a normal physiological process where new capillary branches sprout from preexisting blood vessels. Excessive angiogenesis can lead to the development of cancer, atherosclerosis, rheumatoid arthritis, etc. Anthocyanin is known for inhibiting angiogenesis in human endothelial cells. Quercetin has also shown an inhibitory effect when it was administered to the chorioallantoic membrane of chicken eggs. However, it has been proven that single flavonoids are less effective compared to when they are combined. This study aimed to assess the combined effect of anthocyanin and quercetin in inhibiting blood vessel growth in a chick embryo chorioallantoic membrane. Anthocyanin, quercetin, and their combination were each prepared with a concentration of 15 mg/L. The treatments were administered to seven-day-old chicken eggs and dimethyl sulfoxide served as the positive control. The appearance of each chrorioallantoic membrane was evaluated using the software ImageJ through calculation of fractal dimension values three days after the treatments were introduced. The values were then statistically analyzed through Kruskal-Wallis where it was found out that there was no significant difference between all treatments when compared to the controls. Of the three flavonoid treatments, the combination of anthocyanin and quercetin appeared to be the most effective in reducing blood vessel growth. This shows that the combination of anthocyanin and quercetin is more efficient in inhibiting angiogenesis. This treatment could be a novel source of treating pathological angiogenesis.