An exploratory study on the anti-Angiogenic Property of Ficus benjamina Crude Methanolic Leaf Extract Using Duck (Anas platyrhynchos) Chorioallantoic Membrane (CAM) Assay

LYZ THERESE D. CAINGLET1, AUBREY BEATRICE B. CARNAJE1, ANDREI A. YANSON1, ATHENES JOY P. ABAN1, and RICHIE G. BAYURAN2
1Philippine Science High School – Western Visayas Campus, Bito-on, Jaro, Iloilo City 5000, Department of Science and Technology – Science Education Institute, Philippines
2Saint Gabriel College, Kalibo, Aklan


Abstract
Anti-angiogenesis is the inhibition of the growth of blood vessels. Ficus benjamina is a tree classified under the Moraceae family, and can be frequently seen in households in the Philippines where it commonly grows and is native to Asian countries. The duck chorioallantoic membrane (CAM) assays were treated with positive control (Doxycycline), negative control (Distilled Water), and crude methanolic leaf extract of Ficus benjamina in 3 different concentrations (100 mg/disc, 10 mg/disc, 1 mg/disc). After 72 hours of treatment, the following parameters: total number of branches and total branches length (in pixels), were measured using the ImageJ software. Results show that the values for both parameters of blood vessel growth in CAM assays treated with Treatment 1 (100 mg/disc) were comparable to that of the positive control (Doxycycline). Therefore, the crude methanolic extract of Ficus benjamina exhibits strong anti-angiogenic activity.

Keywords: anti-angiogenesis, CAM Assay, Ficus benjamina, Moraceae, ImageJ

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